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KMID : 1141020160160010069
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Journal of Applied Oriental Medicine
2016 Volume.16 No. 1 p.69 ~ p.78
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Free radical scavenging effect of water extract from Bansi (Diospyroskaki), Cheongdo flat persimmon
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Kim So-Young
Park Hae-Jin
Kim Mi-Ryeo
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Abstract
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Objective : In this study, we investigated the antioxidative capacity of water extract from Bansi (Diospyroskaki.CBS),seedlesspersimmonproducedinCheongdo.
Methods : Total polyphenol and flavonoid contents in CBS were determined by spectrophotometric assay with tannic acid and rutin, as standards. Also DPPH, ABTS, SOD-like scavenging activity, hydroxyl scavenging capacity and reducing power of CBS according to the concentration (0.1~5 mg/ml) were measured invitro.
Results : The polyphenol and flavonoid contents of CBS were 23.27±0.84 mg/TAEg and 19.7±0.32 mg/REg respectively. The antioxidant effect of CBS concentrations (5, 1, 0.5, 0.1 mg/ml) on various antioxidative markers (DPPH, SOD-like, hydroxyl radical scavenging activity, ABTS and reducing power) increased in dose-dependent manners, respectively. Then, DPPH and SOD-like scavenging activities in CBS-treated group (1 and 5 mg/ml) were detected more than 70% compared to those of positive control (BHA)-treated group. In particular, ABTS and reducing power activity in the CBS high dose-treated group (5 mg/ml) significantly increased compared to those of positive control (BHA)-treated group.
Conclusion: These results suggest that the Cheongdo Bansi, could be used as a natural antioxidant for functional food and industrial products.
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KEYWORD
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Antioxidant, Cheongdo Bansi, water extract, DPPH, SOD-like scavenging activity
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